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pseap2 control plasmid  (TaKaRa)


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    Structured Review

    TaKaRa pseap2 control plasmid
    Pseap2 Control Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pseap2+control/pSEAP2-Control+Vector/us12442004-399-20-22
    Average 95 stars, based on 292 article reviews
    pseap2 control plasmid - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Vectors for animal cells and use thereof
    Article Snippet: In order to check whether the constructed L36a-CYHR gene confers the resistance to cycloheximide on animal cells, transfection experiment into Chinese hamster derived CHO (DHFR) cells (ATCC, CRL-9096, referred to hereinafter as “CHO cells”) was performed. .. Specifically, 1 μg of the plasmid pCI-neo-L36 or pCI-neo was co-transfected together with 0.1 μg of plasmid pSEAP2-control (Clontech, 6052-1) containing secretory alkaline phosphatase SEAP into the CHO cells. ..

    Article Title: Vectors for animal cells and utilization thereof
    Article Snippet: In order to check whether the constructed L36a-CYHR gene confers the resistance to cycloheximide on animal cells, transfection experiment into Chinese hamster derived CHO (DHFR) cells (ATCC, CRL-9096, referred to hereinafter as “CHO cells”) was performed. .. Specifically, 1 μg of the plasmid pCI-neo-L36 or pCI-neo was co-transfected together with 0.1 μg of plasmid pSEAP2-control (Clontech, 6052-1) containing secretory alkaline phosphatase SEAP into the CHO cells. ..

    Article Title: Tumor-derived fibulin-3 activates pro-invasive NF-kappa B signaling in glioblastoma cells and their microenvironment
    Article Snippet: The NF-κB reporter plasmid pGL4.32/luc2P/NF-κB-RE was from Promega (Madison WI). .. The SEAP reporter plasmid was generated by removing the luciferase sequence in plasmid pGL4.32 and inserting the sequence of secreted alkaline phosphatase from plasmid pSEAP2-Control (Clontech, Mountain View CA). .. A plasmid carrying the cDNA for the degradation-resistant, super-repressor form of IκBα (pCMX-IκBα-M) was purchased from Addgene (Cambridge, MA).

    Article Title: Tumor-derived fibulin-3 activates pro-invasive NF-κB signaling in glioblastoma cells and their microenvironment.
    Article Snippet: The NF-κB reporter plasmid pGL4.32/luc2P/NF-κB-RE was from Promega (Madison, WI, USA). .. The SEAP reporter plasmid was generated by removing the luciferase sequence in plasmid pGL4.32 and inserting the sequence of secreted alkaline phosphatase from plasmid pSEAP2-Control (Clontech, Mountain View, CA, USA). .. A plasmid carrying the cDNA for the degradation-resistant, super-repressor form of IκBα61 (pCMX-IκBα-M) was purchased from Addgene (Cambridge, MA, USA).

    Article Title: Vector for genetically modifying non-human animals
    Article Snippet: Suspended in one milliliter of pig extender medium (purchased from Merck, Germany, Ref.N.R.13515/0001—dilute mixture M3 for boar sperm), fifteen million sperm cells were incubated with five micrograms of the linker antibody for forty minutes at room temperature with intermittent shaking in between. .. After washing the sperm-linker mixture once with pig extender medium and finally resuspending the mixture in 1.5 ml of the same medium, five micrograms of the plasmid pSEAP2-control (FIG. 12, Clontech Laboratories, Inc., Cat. # 6052-1) were added and incubated with the mixture for forty minutes at room temperature. ..

    Article Title: Vectors for animal cells and use thereof
    Article Snippet: .. The plasmid pSEAP2-control was digested with SalI and blunted with Klenow fragment, and then the BglII linker (5′-CAGATCTG-3′, Takara Shuzo Co., Ltd., 4621P) was ligated. ..

    Article Title: Omega-3 Fatty Acids Inhibit Endoplasmic Reticulum (ER) Stress in Human Coronary Artery Endothelial Cells
    Article Snippet: Newborn calf serum (NCS) was purchased from Hyclone (Logan, UT). .. Lipofectamine was purchased from Invitrogen (Waltham, MA), and the plasmid pSEAP2.control (expressing secreted alkaline phosphatase (SAP)) and the chemiluminescent SAP substrate disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate (CSPD) were purchased from Clone Tech (Palo Alto, CA). .. All the other reagents were purchased from either Termo Fisher Scientifc or Sigma-Aldrich.

    Article Title: Method and composition for genetically modifying non-human cells and animals
    Article Snippet: Suspended in one milliliter of pig extender medium (purchased from Merck, Germany, Ref.N.R.13515/0001—dilute mixture M3 for boar sperm), fifteen million sperm cells were incubated with five micrograms of the linker antibody for forty minutes at room temperature with intermittent shaking in between. .. After washing the sperm-linker mixture once with pig extender medium and finally resuspending the mixture in 1.5 ml of the same medium, five micrograms of the plasmid pSEAP2-control (FIG. 7, Clontech Laboratories, Inc., Cat. #6052-1) were added and incubated with the mixture for forty minutes at room temperature. ..

    Generated:

    Article Title: Tumor-derived fibulin-3 activates pro-invasive NF-kappa B signaling in glioblastoma cells and their microenvironment
    Article Snippet: The NF-κB reporter plasmid pGL4.32/luc2P/NF-κB-RE was from Promega (Madison WI). .. The SEAP reporter plasmid was generated by removing the luciferase sequence in plasmid pGL4.32 and inserting the sequence of secreted alkaline phosphatase from plasmid pSEAP2-Control (Clontech, Mountain View CA). .. A plasmid carrying the cDNA for the degradation-resistant, super-repressor form of IκBα (pCMX-IκBα-M) was purchased from Addgene (Cambridge, MA).

    Article Title: Tumor-derived fibulin-3 activates pro-invasive NF-κB signaling in glioblastoma cells and their microenvironment.
    Article Snippet: The NF-κB reporter plasmid pGL4.32/luc2P/NF-κB-RE was from Promega (Madison, WI, USA). .. The SEAP reporter plasmid was generated by removing the luciferase sequence in plasmid pGL4.32 and inserting the sequence of secreted alkaline phosphatase from plasmid pSEAP2-Control (Clontech, Mountain View, CA, USA). .. A plasmid carrying the cDNA for the degradation-resistant, super-repressor form of IκBα61 (pCMX-IκBα-M) was purchased from Addgene (Cambridge, MA, USA).

    Luciferase:

    Article Title: Tumor-derived fibulin-3 activates pro-invasive NF-kappa B signaling in glioblastoma cells and their microenvironment
    Article Snippet: The NF-κB reporter plasmid pGL4.32/luc2P/NF-κB-RE was from Promega (Madison WI). .. The SEAP reporter plasmid was generated by removing the luciferase sequence in plasmid pGL4.32 and inserting the sequence of secreted alkaline phosphatase from plasmid pSEAP2-Control (Clontech, Mountain View CA). .. A plasmid carrying the cDNA for the degradation-resistant, super-repressor form of IκBα (pCMX-IκBα-M) was purchased from Addgene (Cambridge, MA).

    Article Title: Tumor-derived fibulin-3 activates pro-invasive NF-κB signaling in glioblastoma cells and their microenvironment.
    Article Snippet: The NF-κB reporter plasmid pGL4.32/luc2P/NF-κB-RE was from Promega (Madison, WI, USA). .. The SEAP reporter plasmid was generated by removing the luciferase sequence in plasmid pGL4.32 and inserting the sequence of secreted alkaline phosphatase from plasmid pSEAP2-Control (Clontech, Mountain View, CA, USA). .. A plasmid carrying the cDNA for the degradation-resistant, super-repressor form of IκBα61 (pCMX-IκBα-M) was purchased from Addgene (Cambridge, MA, USA).

    Sequencing:

    Article Title: Tumor-derived fibulin-3 activates pro-invasive NF-kappa B signaling in glioblastoma cells and their microenvironment
    Article Snippet: The NF-κB reporter plasmid pGL4.32/luc2P/NF-κB-RE was from Promega (Madison WI). .. The SEAP reporter plasmid was generated by removing the luciferase sequence in plasmid pGL4.32 and inserting the sequence of secreted alkaline phosphatase from plasmid pSEAP2-Control (Clontech, Mountain View CA). .. A plasmid carrying the cDNA for the degradation-resistant, super-repressor form of IκBα (pCMX-IκBα-M) was purchased from Addgene (Cambridge, MA).

    Article Title: Tumor-derived fibulin-3 activates pro-invasive NF-κB signaling in glioblastoma cells and their microenvironment.
    Article Snippet: The NF-κB reporter plasmid pGL4.32/luc2P/NF-κB-RE was from Promega (Madison, WI, USA). .. The SEAP reporter plasmid was generated by removing the luciferase sequence in plasmid pGL4.32 and inserting the sequence of secreted alkaline phosphatase from plasmid pSEAP2-Control (Clontech, Mountain View, CA, USA). .. A plasmid carrying the cDNA for the degradation-resistant, super-repressor form of IκBα61 (pCMX-IκBα-M) was purchased from Addgene (Cambridge, MA, USA).

    Incubation:

    Article Title: Vector for genetically modifying non-human animals
    Article Snippet: Suspended in one milliliter of pig extender medium (purchased from Merck, Germany, Ref.N.R.13515/0001—dilute mixture M3 for boar sperm), fifteen million sperm cells were incubated with five micrograms of the linker antibody for forty minutes at room temperature with intermittent shaking in between. .. After washing the sperm-linker mixture once with pig extender medium and finally resuspending the mixture in 1.5 ml of the same medium, five micrograms of the plasmid pSEAP2-control (FIG. 12, Clontech Laboratories, Inc., Cat. # 6052-1) were added and incubated with the mixture for forty minutes at room temperature. ..

    Article Title: Method and composition for genetically modifying non-human cells and animals
    Article Snippet: Suspended in one milliliter of pig extender medium (purchased from Merck, Germany, Ref.N.R.13515/0001—dilute mixture M3 for boar sperm), fifteen million sperm cells were incubated with five micrograms of the linker antibody for forty minutes at room temperature with intermittent shaking in between. .. After washing the sperm-linker mixture once with pig extender medium and finally resuspending the mixture in 1.5 ml of the same medium, five micrograms of the plasmid pSEAP2-control (FIG. 7, Clontech Laboratories, Inc., Cat. #6052-1) were added and incubated with the mixture for forty minutes at room temperature. ..

    Expressing:

    Article Title: Omega-3 Fatty Acids Inhibit Endoplasmic Reticulum (ER) Stress in Human Coronary Artery Endothelial Cells
    Article Snippet: Newborn calf serum (NCS) was purchased from Hyclone (Logan, UT). .. Lipofectamine was purchased from Invitrogen (Waltham, MA), and the plasmid pSEAP2.control (expressing secreted alkaline phosphatase (SAP)) and the chemiluminescent SAP substrate disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate (CSPD) were purchased from Clone Tech (Palo Alto, CA). .. All the other reagents were purchased from either Termo Fisher Scientifc or Sigma-Aldrich.



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